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Rapid and sensitive determination of free fatty acids based on in-source microdroplet-driven derivatization coupled with high-resolution mass spectrometry.
Cui, Jiaqi; Zhang, Jianxun; Sun, Shihao; Fan, Wu; Xi, Hui; Xu, Xiujuan; Ji, Lingbo; Zhang, Shusheng; Wang, Dingzhong; Zhao, Wuduo.
Affiliation
  • Cui J; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China.
  • Zhang J; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China.
  • Sun S; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China.
  • Fan W; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China.
  • Xi H; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China.
  • Xu X; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China.
  • Ji L; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China.
  • Zhang S; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; Center of Advanced Analysis and Gene Sequencing, Zhengzhou University, Zhengzhou, 450001, China.
  • Wang D; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China. Electronic address: wdz426@163.com.
  • Zhao W; Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; Center of Advanced Analysis and Gene Sequencing, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: zhaowuduo@163.com.
Anal Chim Acta ; 1278: 341717, 2023 Oct 16.
Article in En | MEDLINE | ID: mdl-37709460
ABSTRACT
Accurate and sensitive measurements of free fatty acids (FFAs) in biological samples are valuable for diagnosing and prognosing diseases. In this study, an in-source microdroplet derivation strategy combined with high-resolution mass spectrometry was developed to analyze FFAs in lipid extracts of biological samples directly. FFAs were rapidly derivated with 2-picolylamine (PA) in the microdroplet which is derived by electrospray. With the proposed method, twelve typical FFAs were determined reliably with high sensitivity and acceptable linearities (R2 ≥ 0.94). The LODs and LOQs for the twelve FFAs were 9-76 pg mL-1 and 30-253 pg mL-1, respectively. The developed method was applied to analyze the alteration of FFAs in liver and kidney samples of rats induced by perfluorooctane sulfonate (PFOS) exposure. The good results demonstrate that the established analysis technique is dependable and has promising applications in detecting FFAs associated with complex biological samples.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fatty Acids, Nonesterified / Kidney Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Anal Chim Acta Year: 2023 Document type: Article Affiliation country: China
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fatty Acids, Nonesterified / Kidney Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Anal Chim Acta Year: 2023 Document type: Article Affiliation country: China