A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets.

Kulcsár, Péter István; Tálas, András; Ligeti, Zoltán; Tóth, Eszter; Rakvács, Zsófia; Bartos, Zsuzsa; Krausz, Sarah Laura; Welker, Ágnes; Végi, Vanessza Laura; Huszár, Krisztina; Welker, Ervin

Kulcsár, Péter István; Tálas, András; Ligeti, Zoltán; Tóth, Eszter; Rakvács, Zsófia; Bartos, Zsuzsa; Krausz, Sarah Laura; Welker, Ágnes; Végi, Vanessza Laura; Huszár, Krisztina; Welker, Ervin.

Affiliation

Kulcsár PI; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Tálas A; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Ligeti Z; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Tóth E; Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
Rakvács Z; Doctoral School of Multidisciplinary Medical Science, University of Szeged, Szeged, Hungary.
Bartos Z; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Krausz SL; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Welker Á; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Végi VL; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
Huszár K; Biospiral-2006 Ltd, Szeged, Hungary.
Welker E; School of Ph.D. Studies, Semmelweis University, Budapest, Hungary.

Nat Commun ; 14(1): 5746, 2023 09 16.

Article in En | MEDLINE | ID: mdl-37717069

Subject(s)

Engineering; Streptococcus pyogenes; Streptococcus pyogenes/genetics

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptococcus pyogenes / Engineering Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2023 Document type: Article Affiliation country: Hungary

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google