LncRNA H19 Inhibits Keratinocyte Cell Proliferation and Migration by Targeting miR-17-5p/RUNX1 Axis in Chronic Wounds.
J Burn Care Res
; 45(2): 366-372, 2024 Mar 04.
Article
in En
| MEDLINE
| ID: mdl-37742288
ABSTRACT
The migration and proliferation of keratinocytes are critical for re-epithelization during chronic wound healing. Runt-related transcription factor 1 (RUNX1) has been indicated to repress keratinocyte proliferation. Nonetheless, the potential molecular mechanism of RUNX1 in regulating keratinocyte proliferation and migration remains unclear. Cell counting kit-8 and wound-healing assays were implemented for examining keratinocyte viability and migration, respectively. Western blotting and real-time quantitative polymerase chain reaction were utilized for quantifying protein and RNA levels. Luciferase reporter assay was employed for verifying the interaction between RUNX1, miR-17-5p, and long noncoding RNA H19. The results showed that RUNX1 depletion promoted keratinocyte proliferation and migration and repressed extracellular matrix degradation. Mechanistically, H19 upregulated RUNX1 expression by competitively absorbing miR-17-5p. Rescue experiments revealed that overexpressing RUNX1 reversed H19 silencing-mediated effects on the phenotypes of keratinocytes. In conclusion, H19 knockdown promotes keratinocyte proliferation and migration and suppresses extracellular matrix degradation via the miR-17-5p/RUNX1 axis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Keratinocytes
/
MicroRNAs
/
Core Binding Factor Alpha 2 Subunit
/
RNA, Long Noncoding
Limits:
Humans
Language:
En
Journal:
J Burn Care Res
Journal subject:
TRAUMATOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
China