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Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites.
Atkinson, Laura; Lee, Jack Cd; Lennon, Alexander; Shah, Divya; Storey, Nathaniel; Morfopoulou, Sofia; Harris, Kathryn A; Breuer, Judy; Brown, Julianne R.
Affiliation
  • Atkinson L; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
  • Lee JC; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
  • Lennon A; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
  • Shah D; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
  • Storey N; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
  • Morfopoulou S; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
  • Harris KA; Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, UK.
  • Breuer J; Royal London Hospital, Barts Health NHS Trust, Department of Virology, London, UK.
  • Brown JR; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
Heliyon ; 9(9): e19854, 2023 Sep.
Article in En | MEDLINE | ID: mdl-37809666
ABSTRACT
Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies / Guideline Language: En Journal: Heliyon Year: 2023 Document type: Article Affiliation country: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies / Guideline Language: En Journal: Heliyon Year: 2023 Document type: Article Affiliation country: United kingdom