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Dielectrophoretic profiling of erythrocytes to study the impacts of metabolic stress, temperature, and storage duration utilizing a point-and-planar microdevice.
Oladokun, Raphael; Adekanmbi, Ezekiel O; An, Vanessa; Gangavaram, Isha; Srivastava, Soumya K.
Affiliation
  • Oladokun R; Department of Chemical and Biomedical Engineering, West Virginia University, 1306 Evansdale Dr., PO Box 6102, Morgantown, WV, 26506-6102, USA.
  • Adekanmbi EO; Intel Corp, Chandler, AZ, USA.
  • An V; Summer 2022 High School Intern, Department of Chemical and Biomedical Engineering, West Virginia University, Morgantown, WV, USA.
  • Gangavaram I; Summer 2022 High School Intern, Department of Chemical and Biomedical Engineering, West Virginia University, Morgantown, WV, USA.
  • Srivastava SK; Department of Chemical and Biomedical Engineering, West Virginia University, 1306 Evansdale Dr., PO Box 6102, Morgantown, WV, 26506-6102, USA. soumya.srivastava@mail.wvu.edu.
Sci Rep ; 13(1): 17281, 2023 10 12.
Article in En | MEDLINE | ID: mdl-37828082
Dielectrophoresis (DEP) is widely utilized for trapping and sorting various types of cells, including live and dead cells and healthy and infected cells. This article focuses on the dielectric characterization of erythrocytes (red blood cells or RBCs) by quantifying DEP crossover frequency using a novel point-and-planar microwell device platform. Numerical simulations using COMSOL Multiphysics software demonstrate that the distribution of the DEP force is influenced by factors such as the shape of the point electrode, spacing between the point and planar electrodes, and the type of bioparticle being investigated. The dependency on electrode spacing is experimentally evaluated by analyzing the DEP crossover response of erythrocytes. Furthermore, the results are validated against the traditional electrical characterization technique called electrorotation, which typically requires laborious fabrication and operation using quadrupole electrodes. Other significant factors, including erythrocyte storage age and the changes in cell properties over time since collection, osmolarity, and temperature, are also assessed to determine the optimal conditions for erythrocyte characterization. The findings indicate a significant difference between fresh and stored erythrocyte samples (up to 4 days), highlighting the importance of maintaining an isotonic medium for cell storage.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stress, Physiological / Erythrocytes Language: En Journal: Sci Rep Year: 2023 Document type: Article Affiliation country: United States Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stress, Physiological / Erythrocytes Language: En Journal: Sci Rep Year: 2023 Document type: Article Affiliation country: United States Country of publication: United kingdom