[Molecular Mechanism of Busulfan Inhibiting the Malignant Biological Characteristics of Multiple Myeloma Cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 31(5): 1426-1431, 2023.
Article
in Zh
| MEDLINE
| ID: mdl-37846695
ABSTRACT
OBJECTIVE:
To investigate the effect of Busulfan on the malignant biological characteristics of multiple myeloma cells and explore the molecular mechanism.METHODS:
Multiple myeloma RPMI8226 cells were treated with Busulfan at different concentrations. Cell proliferation activity was detected by MMT assay, and cell apoptosis was detected by flow cytometry with Annexin V/PI double staining. Real-time quantitative PCR was used to detect the mRNA expression of apoptotic regulatory molecules BaxãBcl-2 and Wnt3a/ß-catenin pathway, and Western blot was used to detect the expression changes of Bax, Bcl-2 and Wnt3a/ß-catenin pathway protein.RESULTS:
Busulfan inhibited cell proliferation and induced apoptosis of myeloma RPMI8226 cells (P<0.05). After treatment with Busulfan at different concentrations for 48 h, the expression of anti-apoptotic protein Bcl-2 was decreased, the expression of pro-apoptotic protein Bax was up-regulated, and the activation of Wnt3a/ß-catenin signaling pathway was inhibited to induce programmed death of RPMI8226 cells (P<0.05).CONCLUSION:
Busulfan can inhibit the malignant biological characteristics of myeloma RPMI 8226 cells, and its mechanism may be related to regulating the expression of Bcl-2 family proteins and inhibiting the activation of Wnt3a/ß-catenin signaling pathway.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
Zh
Journal:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Journal subject:
HEMATOLOGIA
Year:
2023
Document type:
Article
Affiliation country:
China