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A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium.
Morone, Nobuhiro; Martin, Maria Guerra; Goggin, Patricia; Czymmek, Kirk J; Mennella, Vito; Gonzalez, Jaime Llodra.
Affiliation
  • Morone N; Electron Microscopy and Ultrastructural Pathology Facility, Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK. nm669@mrc-tox.cam.ac.uk.
  • Martin MG; Electron Microscopy and Ultrastructural Pathology Facility, Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK.
  • Goggin P; Biomedical Imaging Facility, Laboratory and Pathology Block, Southampton General Hospital, Southampton, UK.
  • Czymmek KJ; Donald Danforth Plant Science Center, Department of Biology, Saint Louis University, Saint Louis, MO, USA.
  • Mennella V; Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK.
  • Gonzalez JL; Electron Microscopy and Ultrastructural Pathology Facility, Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK.
Methods Mol Biol ; 2725: 121-129, 2024.
Article in En | MEDLINE | ID: mdl-37856021
ABSTRACT
Volume electron microscopy technologies such as serial block face scanning electron microscopy (SBF-SEM) allow the characterization of tissue organization and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Imaging, Three-Dimensional / Volume Electron Microscopy Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: United kingdom
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Imaging, Three-Dimensional / Volume Electron Microscopy Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: United kingdom