A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium.
Methods Mol Biol
; 2725: 121-129, 2024.
Article
in En
| MEDLINE
| ID: mdl-37856021
ABSTRACT
Volume electron microscopy technologies such as serial block face scanning electron microscopy (SBF-SEM) allow the characterization of tissue organization and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Imaging, Three-Dimensional
/
Volume Electron Microscopy
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
United kingdom