Improved Culture Methods for Human Coronaviruses HCoV-OC43, HCoV-229E, and HCoV-NL63.
Curr Protoc
; 3(10): e914, 2023 Oct.
Article
in En
| MEDLINE
| ID: mdl-37882768
ABSTRACT
HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoV-HKU1 are four of the seven known human coronaviruses (HCoVs) and, unlike the highly pathogenic SARS-CoV, MERS-CoV, and SARS-CoV-2, these four so-called seasonal HCoVs generally cause mild upper-respiratory-tract illness. As Biosafety Level 2 (BSL-2) pathogens, the seasonal HCoVs are more accessible and can be used as surrogates for studying the highly pathogenic HCoVs. However, scientists have for many years found these difficult to study because of the lack of a universal culture system and the inability of typical culture methods to yield high-titer infectious stocks. We have developed assays to grow and quantify infectious virus and viral RNA for HCoV-OC43, -229E, and -NL63. We identified which immortalized cell lines should be used to optimize the replication of HCoV-OC43, -229E, and -NL63 in order to generate high titers (Vero E6, Huh-7, and LLC-MK2 cells, respectively). Here we present protocols for improved propagation and quantification of each seasonal HCoV. © 2023 Wiley Periodicals LLC. Basic Protocol 1 Growth of HCoVs Basic Protocol 2 Quantification of HCoV by plaque assay Basic Protocol 3 Quantification of HCoV RNA products of replication Basic Protocol 4 Concentrating HCoVs via ultracentrifugation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Coronavirus 229E, Human
/
Coronavirus OC43, Human
/
Coronavirus NL63, Human
Limits:
Humans
Language:
En
Journal:
Curr Protoc
Year:
2023
Document type:
Article