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Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas.
Steiger, Laura; Baumann, Zora; Keller, Lena; Böni-Schnetzler, Marianne; Donath, Marc Y; Meier, Daniel T.
Affiliation
  • Steiger L; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address: laura.steiger@unibas.ch.
  • Baumann Z; Tumor Heterogeneity, Metastasis and Resistance, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Surgery, University Hospital Basel, Basel, Switzerland.
  • Keller L; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland.
  • Böni-Schnetzler M; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland.
  • Donath MY; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland.
  • Meier DT; Clinic of Endocrinology, Diabetes and Metabolism, University Hospital Basel, Basel, Switzerland; Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address: daniel.zeman@unibas.ch.
STAR Protoc ; 4(4): 102664, 2023 Dec 15.
Article in En | MEDLINE | ID: mdl-37889759
ABSTRACT
Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse pancreas, followed by a detailed explanation of how to isolate and analyze immune cells using full-spectrum flow cytometry.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Islets of Langerhans / Pancreas, Exocrine Limits: Animals Language: En Journal: STAR Protoc Year: 2023 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Islets of Langerhans / Pancreas, Exocrine Limits: Animals Language: En Journal: STAR Protoc Year: 2023 Document type: Article