Preparation and characterization of 5-(4-hydroxy-3-nitrobenzyl)-3-phenyl-2-thiohydantoin, the phenylthiohydantoin derivative of 3-nitrotyrosine.
Biol Chem Hoppe Seyler
; 367(10): 1055-9, 1986 Oct.
Article
in En
| MEDLINE
| ID: mdl-3790253
ABSTRACT
The phenylthiocarbamoyl derivative of 3-nitrotyrosine was synthesized according to the known Edman method and then converted to its phenylthiohydantoin derivative [5-(4-hydroxy-3-nitrobenzyl)-3-phenyl-2-thiohydantion] by incubation in 0.5M HCl for 24 h at room temperature. After drying over P2O5 the chromatographically pure substance could be obtained by double recrystallization from hot acetic acid. It could be established that a shorter incubation time leads to an incomplete conversion and higher temperatures cause polymerization of the product. The compounds could be characterized by thin-layer and high-performance liquid chromatography, melting point, elemental analysis as well as NMR- and absorption spectroscopy.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenylthiohydantoin
/
Hydantoins
Language:
En
Journal:
Biol Chem Hoppe Seyler
Journal subject:
BIOQUIMICA
Year:
1986
Document type:
Article