Oocyte rescue in-vitro maturation does not adversely affect chromosome segregation during the first meiotic division.
Reprod Biomed Online
; 48(1): 103379, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-37919136
ABSTRACT
RESEARCH QUESTION Does rescue in-vitro maturation (IVM) in the presence or absence of cumulus cells, affect the progress of meiosis I, compared with oocytes that mature in vivo? DESIGN:
This prospective study was conducted in a university-affiliated fertility centre. Ninety-five young oocyte donors (mean age 25.57 ± 4.47) with a normal karyotype and no known fertility problems were included. A total of 390 oocytes (116 mature metaphase II [MII] and 274 immature oocytes) were analysed. The immature oocytes underwent rescue IVM in the presence of cumulus cells (CC; IVM+CC; nâ¯=â¯137) or without them (IVM-CC; nâ¯=â¯137), and IVM rate was calculated. Chromosome copy number analysis using next-generation sequencing (NGS) was performed on all rescue IVM oocytes reaching MII as well as those that were mature at the time of initial denudation (in-vivo-matured oocytes [IVO]).RESULTS:
Maturation rates were similar in IVM+CC and IVM-CC oocytes (62.8 versus 71.5%, Pâ¯=â¯0.16). Conclusive cytogenetic results were obtained from 65 MII oocytes from the IVM+CC group, 87 from the IVM-CC group, and 99 from the IVO group. Oocyte euploidy rates for the three groups were similar, at 75.4%, 83.9% and 80.8%, respectively (Pâ¯=â¯0.42).CONCLUSIONS:
The results suggest that culture of germinal vesicle and metaphase I oocytes in the presence of cumulus cells does not improve rates of IVM. In general, the process of rescue IVM does not appear to alter the frequency of oocytes with a normal chromosome copy number.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromosome Segregation
/
In Vitro Oocyte Maturation Techniques
Limits:
Adult
/
Humans
Language:
En
Journal:
Reprod Biomed Online
Journal subject:
MEDICINA REPRODUTIVA
Year:
2024
Document type:
Article