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Residues of sulfoxaflor and its metabolites in floral and extrafloral nectar from Hibiscus rosa-sinensis L. (Malvaceae) with or without co-application of tebuconazole.
Zhou, Hong-Xia; Cheng, Man-Huan; Pan, Juan-Lin; Cui, Peng; Song, Yue-Qin; Yu, Ying; Cao, Jun; Zha, Hong-Guang.
Affiliation
  • Zhou HX; College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China.
  • Cheng MH; Instrumental Analysis Centre, Huangshan University, Huangshan 245041, China.
  • Pan JL; College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China.
  • Cui P; Instrumental Analysis Centre, Huangshan University, Huangshan 245041, China.
  • Song YQ; College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China.
  • Yu Y; College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China.
  • Cao J; Yunnan Key Laboratory of Plant Reproductive Adaption and Evolutionary Ecology, Yunnan University, Kunming 650500, China.
  • Zha HG; College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China. Electronic address: arctos725@outlook.com.
Pestic Biochem Physiol ; 196: 105587, 2023 Nov.
Article in En | MEDLINE | ID: mdl-37945224
ABSTRACT
Systemic pesticide exposure through nectar is a growing global concern linked to loss of insect diversity, especially pollinators. The insecticide sulfoxaflor and the fungicide tebuconazole are currently widely used systemic pesticides which are toxic to certain pollinators. However, their metabolisms in floral or extrafloral nectar under different application methods have not yet been well studied. Hibiscus rosa-sinensis was exposed to sulfoxaflor and tebuconazole via soil drenching and foliar spraying. Sulfoxaflor, tebuconazole, and their main metabolites in floral and extrafloral nectar, soil, and leaves were identified and quantified using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-QqQ MS). The chemical compositions of unexposed and contaminated H. rosa-sinensis floral nectar or extrafloral nectar were compared using regular biochemical methods. The activities of two pesticide detoxifying enzymes, glutathione-s-transferase and nitrile hydratase, in H. rosa-sinensis nectar were examined using LC-MS and spectrophotometry. The floral nectar proteome of H. rosa-sinensis was analysed using high-resolution orbitrap-based MS/MS analysis to screen for sulfoxaflor and tebuconazole detoxifying enzymes. H. rosa-sinensis can absorb sulfoxaflor and tebuconazole through its roots or leaf surfaces and secrete them into floral nectar and extrafloral nectar. Both sulfoxaflor and tebuconazole and their major metabolites were present at higher concentrations in extrafloral nectar than in floral nectar. X11719474 was the dominant metabolite of sulfoxaflor in the nectars we studied. Compared with soil application, more sulfoxaflor and tebuconazole remained in their original forms in floral nectar and extrafloral nectar after foliar application. Sulfoxaflor and tebuconazole exposure did not modify the chemical composition of floral or extrafloral nectar. No active components, including proteins in the nectar, were detected to be able to detoxify sulfoxaflor.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pesticides / Malvaceae / Rosa / Hibiscus Language: En Journal: Pestic Biochem Physiol Year: 2023 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pesticides / Malvaceae / Rosa / Hibiscus Language: En Journal: Pestic Biochem Physiol Year: 2023 Document type: Article Affiliation country: China