Network Pharmacology-Based and Molecular Docking-Based Analysis of Cornus Officinalis for the Treatment of Spinal Cord Injury.

ABSTRACT

PURPOSE: Based on network pharmacology and molecular docking, this study aimed to screen out the active ingredients existing in Cornus officinalis for the treatment of spinal cord injury (SCI) and explore their potential mechanisms. METHODS: We collected the active ingredients of Cornus officinalis and its corresponding target proteins. The target proteins corresponding to Cornus officinalis active ingredients were obtained by the Uniport. The SCI genes were obtained through the GeneCards. The active ingredient-acting target network and the interaction between action targets and a target protein interaction network were built by the String and the CytoScape 3.7.2. The core targets were analyzed by the Metascape. The active components and core targets were verified by the AutoDock. RESULTS: We collected eighteen active ingredients, including tetrahydroalstonine. 390 targets, 50 targets related to SCI were obtained. The Key targrts were AKT1, MAPK1, TNF. Four major signaling pathways are involved, including MAPK pathway. The active components of Cornus officinalis have good affinity with the core targets of SCI. CONCLUSION: Our study summarized the active ingredients of Cornus officinalis and the mechanism of action in the treatment of SCI, providing implications for the development of the active ingredients of Cornus officinalis in the treatment of SCI.