Construction of an arrayed CRISPRi library as a resource for essential gene function studies in <i>Streptococcus mutans</i>.

St Pierre, Jackson; Roberts, Justin; Alam, Mohammad A; Shields, Robert C

Construction of an arrayed CRISPRi library as a resource for essential gene function studies in Streptococcus mutans.

St Pierre, Jackson; Roberts, Justin; Alam, Mohammad A; Shields, Robert C.

Affiliation

St Pierre J; Department of Biological Sciences, Arkansas State University , Jonesboro, Arkansas, USA.
Roberts J; New York Institute of Technology College of Osteopathic Medicine , Jonesboro, Arkansas, USA.
Alam MA; Department of Chemistry & Physics, Arkansas State University , Jonesboro, Arkansas, USA.
Shields RC; University of Arkansas for Medical Sciences , Little Rock, Arkansas, USA.

Microbiol Spectr ; 12(1): e0314923, 2024 Jan 11.

Article in En | MEDLINE | ID: mdl-38054713

Subject(s)

Genes, Essential; Streptococcus mutans; Streptococcus mutans/genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Library; CRISPR-Cas Systems

Key words

CRISPRi; Streptococcus mutans; arrayed mutant library; essential genes; functional genomics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptococcus mutans / Genes, Essential Language: En Journal: Microbiol Spectr Year: 2024 Document type: Article Affiliation country: United States

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