Karyotyping of aneuploid and polyploid plants from low coverage whole-genome resequencing.
BMC Plant Biol
; 23(1): 630, 2023 Dec 08.
Article
in En
| MEDLINE
| ID: mdl-38062348
ABSTRACT
BACKGROUND:
Karyotype, as a basic characteristic of species, provides valuable information for fundamental theoretical research and germplasm resource innovation. However, traditional karyotyping techniques, including fluorescence in situ hybridization (FISH), are challenging and low in efficiency, especially when karyotyping aneuploid and polyploid plants. The use of low coverage whole-genome resequencing (lcWGR) data for karyotyping was explored, but existing methods are complicated and require control samples.RESULTS:
In this study, a new protocol for molecular karyotype analysis was provided, which proved to be a simpler, faster, and more accurate method, requiring no control. Notably, our method not only provided the copy number of each chromosome of an individual but also an accurate evaluation of the genomic contribution from its parents. Moreover, we verified the method through FISH and published resequencing data.CONCLUSIONS:
This method is of great significance for species evolution analysis, chromosome engineering, crop improvement, and breeding.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyploidy
/
Aneuploidy
Language:
En
Journal:
BMC Plant Biol
Journal subject:
BOTANICA
Year:
2023
Document type:
Article
Affiliation country:
China