Carbon-coated copper nanocrystals with enhanced peroxidase-like activity for sensitive colorimetric determination of 2,4-dinitrophenylhydrazine.
Mikrochim Acta
; 191(1): 37, 2023 12 18.
Article
in En
| MEDLINE
| ID: mdl-38110783
ABSTRACT
Carbon-coated copper nanocrystals (CuNCs) with peroxidase-like activity were hydrothermally prepared by using copper acetate, citric acid (CA) and histidine (His) as the precursors. Various shaped CuNCs, including urchin-like, slab-like and spherical appearance were facilely prepared by addition of different amount of NaNO2 in the precursor solutions. When 3,3',5,5'-tetramethylbenzidine (TMB) was used as the substrate, the CuNCs with urchin-like appearance have greatest peroxidase-like activity and their Michaelis-Menten constant (Km) and the maximum rate constant (νmax) are respectively 8.8 and 1.2 times higher than that obtained from horseradish peroxidase (HRP). The production of reactive oxygen species (ROS) was confirmed by radical quenching and electron spin resonance (ESR) tests. Subsequent studies have found that the CuNCs catalyzed color reaction of TMB can be selectively quenched by the environmental pollutant 2,4-dinitrophenylhydrazine (2,4-DNPH). Thus a new colorimetric method for the determination of 2,4-DNPH with a linear range of 0.60-20 µM was developed and a limit of detection (LOD) as low as 0.166 µM was achieved. The results obtained not only reveal the tunability of the peroxidase-like activity of Cu-based nanomaterials, but also provide a new method for the sensitive determination of environmental contaminate.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biomimetic Materials
/
Nanoparticles
Language:
En
Journal:
Mikrochim Acta
Year:
2023
Document type:
Article
Affiliation country:
China