Detection of Multiple Antibiotic Residues in Turkish Pine and Blossom Honeys Using LC-MS/MS Method.
Chem Biodivers
; 21(1): e202301261, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-38116889
ABSTRACT
Due to the high demand for honey, beekeepers often feed the bees with antibiotics to protect honeybees against illnesses; the determination of veterinary drugs and their residues in bee products especially in honey is gaining importance. In this study, commercially available 15 different brands, a total of 22 honey (14 blossoms and 8 pines) samples obtained from 5 chain supermarkets in the city of Bingöl and Diyarbakir, Turkey were analysed for 29 antibiotic residues. These antibiotics belong to 10 different categories, including tetracyclines, aminoglycosides, macrolides, sulfonamides, fluoroquinolones, benzimidazoles, anthelmintic, amphenicols, quinolines, and oxazolidines. For the qualitative and quantitative determination of the antibiotics, a triple quadrupole liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used. A total of 10 out of 22 honey (8 blossom, 57.14 % and 2 pine, 25 %) samples were found to be positive for antibiotics. Among the tested antibiotics, tetracycline, dihydrostreptomycin, streptomycin, erythromycin, and sulfadimidine were detected in the honey samples. Dihydrostreptomycin and sulfadimidine were detected in 6 samples, erythromycin was determined in 4 samples, streptomycin was found in 2 samples, and lastly, tetracycline was detected only in one sample. The highest and the lowest concentrations of antibiotics detected in the samples were dihydrostreptomycin and erythromycin found at the amount of 992.58â
µg/kg and 0.77â
µg/kg respectively. The proposed method was validated with a limit of quantification (LOQ) and limit of detection (LOD) ranging between 0.42 and 3.22â
µg /kg and 0.13-0.97â
µg /kg respectively. Good linearities were also achieved ranging between R2 =0.987 and 0.999.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dihydrostreptomycin Sulfate
/
Honey
Limits:
Animals
Language:
En
Journal:
Chem Biodivers
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2024
Document type:
Article
Affiliation country:
Turkey