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Preparation of two zwitterionic polymer functionalized stationary phases and comparative evaluation under mixed-mode of reversed phase/ hydrophilic interaction/ion exchange chromatography.
Wang, Xiang; Zhu, Tianrun; Wang, Xingrui; Peng, Huanjun; Zhou, Guangming; Peng, Jingdong.
Affiliation
  • Wang X; School of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China.
  • Zhu T; Chengdu University of Technology, Yibin 644000, PR China.
  • Wang X; School of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China.
  • Peng H; School of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China.
  • Zhou G; School of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China. Electronic address: gmzhou@swu.edu.cn.
  • Peng J; School of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China. Electronic address: hxpengjd@swu.edu.cn.
J Chromatogr A ; 1714: 464586, 2024 Jan 11.
Article in En | MEDLINE | ID: mdl-38118242
ABSTRACT
Zwitterions are a promising choice to prepare separation materials because of their hydrophilicity and biocompatibility. We described the preparation of two zwitterionic polymer functionalized stationary phases and evaluation under mixed-mode chromatography. A zwitterionic monomer, S-(4-vinylbenzyl) cysteine (SVC), was synthesized and bonded to silica via reversible addition fragmentation chain transfer (RAFT) polymerization to afford a zwitterionic stationary phase, Sil-SVC. A hydrophobic monomer, N-(4-phenylbutan-2-yl) acrylamide (NPA), was copolymerized with SVC onto the stationary phase (Sil-SVCNPA) for comparison. The stationary phases were characterized with FT-IR, TGA, EA, and zeta-potential measurements. Mobile phase composition (ACN content, pH and salt concentration) was varied to study the retention property. Linear solvation energy relationship and Van't Hoff plot were used to investigate the retention mechanism and how chromatographic conditions influenced it. Both stationary phases showed a mixed-mode of RPLC/HILIC/IEC and satisfactory performance in separating hydrophobic analytes (alkylbenzenes and polycyclic aromatic hydrocarbons), hydrophilic nucleotide and bases, and anions, high column efficiency of 60,000 plates·m-1 was achieved. In summary, zwitterionic polymers are attractive options to prepare stationary phases and the retention property can be easily regulated by copolymer.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymers / Chromatography, Reverse-Phase Language: En Journal: J Chromatogr A Year: 2024 Document type: Article Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymers / Chromatography, Reverse-Phase Language: En Journal: J Chromatogr A Year: 2024 Document type: Article Country of publication: Netherlands