Conversion of radiolabelled ethanolamine plasmalogen into the dimethylethanolamine and choline analogue via transphosphatidylation by phospholipase D from cabbage.
Chem Phys Lipids
; 41(3-4): 349-53, 1986.
Article
in En
| MEDLINE
| ID: mdl-3815628
ABSTRACT
Starting from biosynthetically prepared ethanolamine plasmalogen 14C-labelled in the O-alkenyl moiety, choline and dimethylethanolamine plasmalogen were prepared by transphosphatidylation utilizing phospholipase D from cabbage. Investigation of the time course of the reaction showed that transphosphatidylation was simultaneously accompanied by hydrolysis of both the substrate and the desired product, resulting in a maximum of product yield after 1-3 h under the reaction conditions investigated. Optimal reaction conditions gave yields of 40% and 62% (of total radioactivity) respectively for the purified choline and dimethylethanolamine derivatives.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipase D
/
Phospholipases
/
Brassica
/
Plasmalogens
Limits:
Animals
Language:
En
Journal:
Chem Phys Lipids
Year:
1986
Document type:
Article
Publication country:
IE
/
IRELAND
/
IRLANDA