CD317+ MSCs expanded with chemically defined media have enhanced immunological anti-inflammatory activities.
Stem Cell Res Ther
; 15(1): 2, 2024 01 02.
Article
in En
| MEDLINE
| ID: mdl-38169422
ABSTRACT
BACKGROUND:
Although both preclinical and clinical studies have shown the great application potential of MSCs (mesenchymal stem/stromal cells) in treating many kinds of diseases, therapeutic inconsistency resulting from cell heterogeneity is the major stumbling block to their clinical applications. Cell population diversity and batch variation in the cell expansion medium are two major inducers of MSC heterogeneity.METHODS:
Cell population diversity was investigated through single-cell RNA sequencing analysis of human MSCs derived from the umbilical cord and expanded with fully chemically defined medium in the current study. Then, the MSC subpopulation with enhanced anti-inflammatory effects was studied in vitro and in vivo.RESULTS:
Our data showed that MSCs contain different populations with different functions, including subpopulations with enhanced functions of exosome secretion, extracellular matrix modification and responses to stimuli (regeneration and immune response). Among them, CD317+ MSCs have improved differentiation capabilities and enhanced immune suppression activities. Underlying mechanism studies showed that higher levels of TSG6 confer enhanced anti-inflammatory functions of CD317+ MSCs.CONCLUSIONS:
Thus, CD317+ MSCs might be a promising candidate for treating immunological disorder-related diseases.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mesenchymal Stem Cell Transplantation
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
Stem Cell Res Ther
Year:
2024
Document type:
Article