Cross-Neutralization Activities of Antibodies against 18 Lyssavirus Glycoproteins.
Jpn J Infect Dis
; 77(3): 169-173, 2024 May 23.
Article
in En
| MEDLINE
| ID: mdl-38171846
ABSTRACT
Some lyssaviruses, including the rabies virus (RABV), cause lethal neurological symptoms in humans. However, the efficacy of commercial vaccines has only been evaluated against RABV. To assess cross-reactivity among lyssaviruses, including RABV, sera from rabbits inoculated with human and animal RABV vaccines and polyclonal antibodies from rabbits immunized with expression plasmids of the glycoproteins of all 18 lyssaviruses were prepared, and cross-reactivity was evaluated via virus-neutralization tests using Duvenhage lyssavirus (DUVV), European bat lyssavirus-1 (EBLV-1), Mokola lyssavirus (MOKV), Lagos bat lyssavirus (LBV), and RABV. The sera from rabbits inoculated with RABV vaccines showed cross-reactivity with EBLV-1 and DUVV, both belonging to phylogroup I. However, reactivity with MOKV and LBV in phylogroup II was notably limited or below the detection level. Next, we compared the cross-reactivity of the polyclonal antibodies against all lyssavirus glycoproteins. Polyclonal antibodies had high virus-neutralization titers against the same phylogroup but not different phylogroups. Our findings indicate that a new vaccine should be developed for pre- and post-exposure prophylaxis against lyssaviral infections.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neutralization Tests
/
Glycoproteins
/
Lyssavirus
/
Cross Reactions
/
Antibodies, Neutralizing
/
Antibodies, Viral
Limits:
Animals
/
Humans
Language:
En
Journal:
Jpn J Infect Dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2024
Document type:
Article
Affiliation country:
Japan