From Sea to Soil: Marine Bacillus subtilis enhancing chickpea production through in vitro and in vivo plant growth promoting traits.

ABSTRACT

Various strategies are used to augment agricultural output in response to the escalating food requirements stemming from population expansion. Out of various strategies, the use of plant growth-promoting bacteria (PGPB) has shown promise as a viable technique in implementing new agricultural practices. The study of PGPB derived from rhizospheric soil is extensive, but there is a need for more exploration of marine microorganisms. The present research aims to investigate the potential of marine microorganisms as promoters of plant growth. The marine microbe Bacillus subtilis used in current study has been discovered as a possible plant growth-promoting bacterium (PGPB) as it showed ability to produce ammonia, solubilize potassium and phosphate, and was able to colonize chickpea roots. Bacillus subtilis exhibited a 40% augmentation in germination. A talc-based bio-formulation was prepared using Bacillus subtilis, and pot experiment was done under two conditions control (T1) and Bacillus treated (T2). In the pot experiment, the plant weight with Bacillus treatment increased by 14.17%, while the plant height increased by 13.71% as compared to control. It also enhanced the chlorophyll content of chickpea and had a beneficial influence on stress indicators. Furthermore, it was noted that it enhanced the levels of nitrogen, potassium, and phosphate in the soil improving soil quality. The findings showed that B. subtilis functioned as a plant growth-promoting bacteria (PGPB) to enhance the overall development of chickpea.