Antibacterial mechanism of inosine against Alicyclobacillus acidoterrestris.
J Food Sci
; 89(2): 1187-1195, 2024 Feb.
Article
in En
| MEDLINE
| ID: mdl-38204435
ABSTRACT
Inosine could potentially become a novel antibacterial agent against Alicyclobacillus acidoterrestris as low doses of inosine can prevent its contamination. However, until now the antibacterial mechanism of inosine targeting A. acidoterrestris is still unknown. In this study, to unravel the mechanism of inosine against A. acidoterrestris puzzle, the effects of inosine on bacterial surface hydrophobicity, intracellular protein content, cell membrane damage extent, and permeability of the A. acidoterrestris were investigated. The results showed that inosine can effectively inhibit the growth and reproduction of A. acidoterrestris by destroying the integrity of cell membrane and increasing its permeability, causing the leakage of intracellular nutrients. Furthermore, the interaction networks of inosine target proteins were analyzed. The interaction networks further revealed that damage to bacterial cell membranes might be relevant to inosine's effect on bacterial DNA replication and cell energy metabolism through regulating nucleotide synthesis and metabolism and the activity of translation initiation factors. Finally, the antibacterial mechanism of inosine against A. acidoterrestris was proposed.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Alicyclobacillus
/
Anti-Bacterial Agents
Language:
En
Journal:
J Food Sci
Year:
2024
Document type:
Article
Affiliation country:
China