Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies.
Science
; 383(6680): eadf2341, 2024 01 19.
Article
in En
| MEDLINE
| ID: mdl-38236959
ABSTRACT
Liquid biopsies enable early detection and monitoring of diseases such as cancer, but their sensitivity remains limited by the scarcity of analytes such as cell-free DNA (cfDNA) in blood. Improvements to sensitivity have primarily relied on enhancing sequencing technology ex vivo. We sought to transiently augment the level of circulating tumor DNA (ctDNA) in a blood draw by attenuating its clearance in vivo. We report two intravenous priming agents given 1 to 2 hours before a blood draw to recover more ctDNA. Our priming agents consist of nanoparticles that act on the cells responsible for cfDNA clearance and DNA-binding antibodies that protect cfDNA. In tumor-bearing mice, they greatly increase the recovery of ctDNA and improve the sensitivity for detecting small tumors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell-Free Nucleic Acids
/
Neoplasms
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Science
Year:
2024
Document type:
Article
Affiliation country:
United States