Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells by WNT Switch Method.
Cells
; 13(2)2024 01 11.
Article
in En
| MEDLINE
| ID: mdl-38247824
ABSTRACT
The differentiation of ESCs into cardiomyocytes in vitro is an excellent and reliable model system for studying normal cardiomyocyte development in mammals, modeling cardiac diseases, and for use in drug screening. Mouse ESC differentiation still provides relevant biological information about cardiac development. However, the current methods for efficiently differentiating ESCs into cardiomyocytes are limiting. Here, we describe the "WNT Switch" method to efficiently commit mouse ESCs into cardiomyocytes using the small molecule WNT signaling modulators CHIR99021 and XAV939 in vitro. This method significantly improves the yield of beating cardiomyocytes, reduces number of treatments, and is less laborious.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retinal Degeneration
/
Vision Disorders
/
Eye Diseases, Hereditary
/
Myocytes, Cardiac
/
Mouse Embryonic Stem Cells
Limits:
Animals
Language:
En
Journal:
Cells
Year:
2024
Document type:
Article
Affiliation country:
United States
Country of publication:
Switzerland