SOX2 and SOX9 Expression in Developing Postnatal Opossum (Monodelphis domestica) Cortex.
Biomolecules
; 14(1)2024 Jan 05.
Article
in En
| MEDLINE
| ID: mdl-38254670
ABSTRACT
(1) Background:
Central nervous system (CNS) development is characterized by dynamic changes in cell proliferation and differentiation. Key regulators of these transitions are the transcription factors such as SOX2 and SOX9. SOX2 is involved in the maintenance of progenitor cell state and neural stem cell multipotency, while SOX9, expressed in neurogenic niches, plays an important role in neuron/glia switch with predominant expression in astrocytes in the adult brain. (2)Methods:
To validate SOX2 and SOX9 expression patterns in developing opossum (Monodelphis domestica) cortex, we used immunohistochemistry (IHC) and the isotropic fractionator method on fixed cortical tissue from comparable postnatal ages, as well as dissociated primary neuronal cultures. (3)Results:
Neurons positive for both neuronal (TUJ1 or NeuN) and stem cell (SOX2) markers were identified, and their presence was confirmed with all methods and postnatal age groups (P4-6, P6-18, and P30) analyzed. SOX9 showed exclusive staining in non-neuronal cells, and it was coexpressed with SOX2. (4)Conclusions:
The persistence of SOX2 expression in developing cortical neurons of M. domestica during the first postnatal month implies the functional role of SOX2 during neuronal differentiation and maturation, which was not previously reported in opossums.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Monodelphis
/
SOX Transcription Factors
/
Neural Stem Cells
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biomolecules
Year:
2024
Document type:
Article
Affiliation country:
Croatia