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Circ-MBOAT2 Regulates Angiogenesis via the miR-495/NOTCH1 Axis and Associates with Myocardial Perfusion in Patients with Coronary Chronic Total Occlusion.
Gao, Wei; Li, Chenguang; Yuan, Jie; Zhang, Youming; Liu, Guobing; Zhang, Jianhui; Shi, Hongcheng; Liu, Haibo; Ge, Junbo.
Affiliation
  • Gao W; Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
  • Li C; National Clinical Research Center for Interventional Medicine, Shanghai 200032, China.
  • Yuan J; Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
  • Zhang Y; National Clinical Research Center for Interventional Medicine, Shanghai 200032, China.
  • Liu G; Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
  • Zhang J; National Clinical Research Center for Interventional Medicine, Shanghai 200032, China.
  • Shi H; Department of Cardiology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China.
  • Liu H; Department of Nuclear Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
  • Ge J; Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Int J Mol Sci ; 25(2)2024 Jan 08.
Article in En | MEDLINE | ID: mdl-38255868
ABSTRACT
Revascularization of coronary chronic total occlusion (CTO) still remains controversial. The factors that impact collateral circulation and myocardial perfusion are of interest. Circular RNA (circRNA) has been shown to regulate the process of angiogenesis. However, the effects of circ-membrane-bound O-acyltransferase domain containing 2 (circ-MBOAT2) on angiogenesis in patients with CTO were unclear. In this study, we evaluated circulating circRNAs and miRNAs in patients with CTO and stable coronary artery disease using high-throughput sequencing. Another cohort of patients were selected to verify the expressions of circ-MBOAT2 and miR-495. The role and mechanism of circ-MBOAT2 in the process of angiogenesis were explored through in vitro and vivo studies. Finally, we came back to a clinical perspective and investigated whether circ-MBOAT2 and miR-495 were associated with the improvement of myocardial perfusion evaluated by single-photon emission computed tomography (SPECT). We found that the expression of circ-MBOAT2 was significantly up-regulated while miR-495 was significantly down-regulated in patients with CTO. The expression of circ-MBOAT2 was negatively correlated with miR-495 in patients with CTO. In an in vitro study, we found that circ-MBOAT2 promoted tube formation and cell migration via the miR-495/NOTCH1 axis in endothelial cells. In an in vivo study, we showed that the inhibition of miR-495 caused the increase in collateral formation in mice after hindlimb ischemia. In a human study, we showed the expressions of circ-MBOAT2 and miR-495 were associated with myocardial perfusion improvement after revascularization of CTO. In conclusion, circ-MBOAT2 regulates angiogenesis via the miR-495/NOTCH1 axis and associates with myocardial perfusion in patients with CTO. Our findings suggest that circ-MBOAT2 and miR-495 may be potential therapeutic targets and prognostic factors for patients with CTO.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Myocardial Reperfusion / MicroRNAs / Coronary Occlusion / Percutaneous Coronary Intervention / RNA, Circular Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals / Humans Language: En Journal: Int J Mol Sci Year: 2024 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Myocardial Reperfusion / MicroRNAs / Coronary Occlusion / Percutaneous Coronary Intervention / RNA, Circular Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals / Humans Language: En Journal: Int J Mol Sci Year: 2024 Document type: Article Affiliation country: China