Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea.
Methods Mol Biol
; 2751: 47-68, 2024.
Article
in En
| MEDLINE
| ID: mdl-38265709
ABSTRACT
The most important advances in our understanding of the viral life cycle, such as genome replication, packaging, transmission, and host interactions, have been made via the development of viral infectious full-length clones. Here, we describe the detailed protocols for the construction of an infectious clone derived from Botrytis virus F (BVF), a mycoflexivirus infecting the plant pathogenic fungus Botrytis cinerea, the determination of the complete sequence of the cloned mycovirus, the preparation of fungal protoplasts, and the transfection of protoplasts using transcripts derived from the BVF infectious clone.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Communicable Diseases
/
Fungal Viruses
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Spain
Country of publication:
United States