The induction of a specific form of cytochrome P-450 (P-450j) by fasting.
Biochem Biophys Res Commun
; 142(3): 1077-83, 1987 Feb 13.
Article
in En
| MEDLINE
| ID: mdl-3827895
ABSTRACT
In previous work we have demonstrated that liver microsomal N-nitrosodimethylamine demethylase (NDMAd) activity is increased in rats by fasting, and we have postulated that this is due to the induction of a specific form of cytochrome P-450. This communication provides evidence for such a hypothesis. Fasting for 24 and 48 h caused 59 and 116% increases, respectively, in NDMAd activity in male rats, and fasting for 48 h caused a 63% increase in female rats. These increases were accompanied by corresponding increases of cytochrome P-450j (P-450ac) determined by immunoblotting. Fasting for 24 and 48 h also increased the mRNA for P-450j by 153 to 250%, as determined by hybridization with a cDNA probe of this cytochrome. The results suggest that fasting affects the gene expression of P-450j.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fasting
/
Cytochrome P-450 Enzyme System
/
Isoenzymes
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1987
Document type:
Article