Step-by-step approach: Stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system.
MethodsX
; 12: 102544, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38283759
ABSTRACT
In vivo extracellular field potential recording is a commonly used technique in modern neuroscience research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery and the application of the eLab/ePulse electrophysiology system in rodent models. This study presents a practical and functional manual guide for surgical electrode implantation in rodent models using the eLab/ePulse electrophysiology system for recording and stimulation purposes to assess neuronal functionality and synaptic plasticity. The evaluation parameters included the input/output function (IO), paired-pulse facilitation or depression (PPF/PPD), long-term potentiation (LTP), and long-term depression (LTD).â¢Provides a detailed picture-guided procedure for conducting in vivo stereotaxic neurosurgery.â¢Specifically covers the insertion of hippocampal electrodes and the recording of evoked extracellular field potentials.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Guideline
Language:
En
Journal:
MethodsX
Year:
2024
Document type:
Article
Affiliation country:
Malaysia