The microbiological outcomes of culture-negative blood specimens using 16S rRNA broad-range PCR sequencing: a retrospective study in a Canadian province from 2018 to 2022.
J Clin Microbiol
; 62(3): e0151823, 2024 03 13.
Article
in En
| MEDLINE
| ID: mdl-38299828
ABSTRACT
Broad-range 16S rRNA PCR and sequencing of 1,183 blood specimens from 853 unique patients yielded an interpretable sequence and bacterial identification in 29%, 16S rRNA amplification with uninterpretable sequences in 53%, and no amplification in 18%. This study highlights the potential utility of this technique in identifying fastidious gram-negative and anaerobic bacteria but the frequent recovery of environmental and contaminant organisms argues for its judicious use. IMPORTANCE The existing literature focuses on its performance compared to blood cultures in patients with sepsis, leaving a gap in the literature regarding other blood specimens in suspected infectious syndrome across the severity spectrum. We aimed to characterize its microbiological outcomes and provide insight into its potential clinical utility.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Ribosomal, 16S
Type of study:
Observational_studies
Limits:
Humans
Country/Region as subject:
America do norte
Language:
En
Journal:
J Clin Microbiol
Year:
2024
Document type:
Article
Affiliation country:
Canada
Country of publication:
United States