Nuclear translocation of Axl contributes to the malignancy of oral cancer cells.
J Dent Sci
; 19(1): 438-447, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-38303797
ABSTRACT
Background/purpose:
Dysregulation of receptor tyrosine kinases is implicated in cancer development. This study aimed to investigate the nuclear translocation of Axl, a membrane protein and receptor tyrosine kinase in cancer malignancy. Materials andmethods:
We examined Axl's entry into the cell nucleus and validated it with the nuclear export inhibitor leptomycin. Transfection experiments with mutated nuclear localization signals were conducted to assess the impact of reduced nuclear Axl levels on cancer cell malignancy. Additionally, we evaluated the effects of decreased nuclear Axl on sensitivity to radiation and cisplatin, a chemotherapeutic drug.Results:
In the present study, we observed nuclear translocation of Axl in cancer cells. Reducing nuclear Axl levels led to a decrease in cancer cell malignancy. This nuclear translocation was further validated using a nuclear export inhibitor, leptomycin. Additionally, transfection experiments with mutated nuclear localization signals confirmed the functional significance of Axl's nuclear localization. Notably, decreased nuclear Axl levels also increased the sensitivity of cancer cells to radiation and cisplatin treatment.Conclusion:
This study suggests that Axl's nuclear translocation plays a significant role in cancer malignancy. Targeting Axl's nuclear localization could offer a potential strategy to inhibit cancer progression and improve the efficacy of radiation and chemotherapy treatments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Dent Sci
Year:
2024
Document type:
Article
Affiliation country:
Taiwan
Country of publication:
Netherlands