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Protocol for neurogenin-2-mediated induction of human stem cell-derived neural progenitor cells.
Guss, Ellen J; Sathe, Laila; Dai, Alexander; Derebenskiy, Tim; Vega, Ana Rodriguez; Eggan, Kevin; Wells, Michael F.
Affiliation
  • Guss EJ; Department of Stem Cell and Regenerative Biology, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
  • Sathe L; Department of Human Genetics, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA.
  • Dai A; Department of Human Genetics, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA.
  • Derebenskiy T; Department of Human Genetics, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA.
  • Vega AR; Department of Human Genetics, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA.
  • Eggan K; Department of Stem Cell and Regenerative Biology, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Wells MF; Department of Human Genetics, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA. Electronic address: mfwells@mednet.ucla.edu.
STAR Protoc ; 5(1): 102878, 2024 Mar 15.
Article in En | MEDLINE | ID: mdl-38335091
ABSTRACT
Human pluripotent stem cell-derived neural progenitor cells (NPCs) are an essential tool for the study of brain development and developmental disorders such as autism. Here, we present a protocol to generate NPCs rapidly and reproducibly from human stem cells using dual-SMAD inhibition coupled with a brief pulse of mouse neurogenin-2 (Ngn2) overexpression. We detail the 48-h induction scheme deployed to produce these cells-termed stem cell-derived Ngn2-accelerated progenitor cells-followed by steps for expansion, purification, banking, and quality assessment. For complete details on the use and execution of this protocol, please refer to Wells et al.1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pluripotent Stem Cells / Neural Stem Cells Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article Affiliation country: United States Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pluripotent Stem Cells / Neural Stem Cells Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article Affiliation country: United States Country of publication: United States