Human iPSC-derived photoreceptor transplantation in the cone dominant 13-lined ground squirrel.
Stem Cell Reports
; 19(3): 331-342, 2024 Mar 12.
Article
in En
| MEDLINE
| ID: mdl-38335965
ABSTRACT
Several retinal degenerations affect the human central retina, which is primarily comprised of cones and is essential for high acuity and color vision. Transplanting cone photoreceptors is a promising strategy to replace degenerated cones in this region. Although this approach has been investigated in a handful of animal models, commonly used rodent models lack a cone-rich region and larger models can be expensive and inaccessible, impeding the translation of therapies. Here, we transplanted dissociated GFP-expressing photoreceptors from retinal organoids differentiated from human induced pluripotent stem cells into the subretinal space of damaged and undamaged cone-dominant 13-lined ground squirrel eyes. Transplanted cell survival was documented via noninvasive high-resolution imaging and immunohistochemistry to confirm the presence of human donor photoreceptors for up to 4 months posttransplantation. These results demonstrate the utility of a cone-dominant rodent model for advancing the clinical translation of cell replacement therapies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retinal Degeneration
/
Induced Pluripotent Stem Cells
Limits:
Animals
/
Humans
Language:
En
Journal:
Stem Cell Reports
Year:
2024
Document type:
Article
Affiliation country:
United States
Country of publication:
United States