Chemical profile of the roots of Clausena lansium and their inhibitory effects of the over-activation in BV-2 microglial cells.
Phytochemistry
; 220: 114008, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38346545
ABSTRACT
From the 95% ethanol aqueous extract of the roots of Clausena lansium, six previously undescribed alkaloids (1, 2a, 2b, 15, 24a, 24b), a pair of prenylated phenylpropenols (26a, 26b), two coumarins (27, 28), and two undescribed sesquiterpenes (37, 38) were isolated and identified using spectroscopic and electron circular dichroism data, together with thirty-two known compounds. The absolute configurations of three alkaloids (3a, 3b, 4a) were determined for the first time. In vitro assay showed that alkaloids 7, 10, 12, 19, and furanocoumarins 34, 35 displayed inhibitory effects on the production of nitric oxide in lipopolysaccharide (LPS)-induced BV-2 microglial cells, which were stronger than that of the minocycline (positive control). RT-PCR results indicated that indizoline (7) could inhibit the expression of pro-inflammatory factors (IL-1ß, TNF-α, and IL-6) in LPS-treated BV-2 cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Alkaloids
/
Clausena
Type of study:
Prognostic_studies
Language:
En
Journal:
Phytochemistry
Year:
2024
Document type:
Article
Country of publication:
United kingdom