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A transcriptomic time-series reveals differing trajectories during pre-floral development in the apex and leaf in winter and spring varieties of Brassica napus.
Jones, D Marc; Hepworth, Jo; Wells, Rachel; Pullen, Nick; Trick, Martin; Morris, Richard J.
Affiliation
  • Jones DM; Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. dr.d.marc.jones@gmail.com.
  • Hepworth J; Computational and Systems Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. dr.d.marc.jones@gmail.com.
  • Wells R; Synthace, The WestWorks, 195 Wood Lane, 4th Floor, London, W12 7FQ, UK. dr.d.marc.jones@gmail.com.
  • Pullen N; Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • Trick M; Department of Biosciences, Durham University, Stockton Road, Durham, DH1 3LE, UK.
  • Morris RJ; Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Sci Rep ; 14(1): 3538, 2024 02 12.
Article in En | MEDLINE | ID: mdl-38347020
ABSTRACT
Oilseed rape (Brassica napus) is an important global oil crop, with spring and winter varieties grown commercially. To understand the transcriptomic differences between these varieties, we collected transcriptomes from apex and leaf tissue from a spring variety, Westar, and a winter variety, Tapidor, before, during, and after vernalisation treatment, until the plants flowered. Large transcriptomic differences were noted in both varieties during the vernalisation treatment because of temperature and day length changes. Transcriptomic alignment revealed that the apex transcriptome reflects developmental state, whereas the leaf transcriptome is more closely aligned to the age of the plant. Similar numbers of copies of genes were expressed in both varieties during the time series, although key flowering time genes exhibited expression pattern differences. BnaFLC copies on A2 and A10 are the best candidates for the increased vernalisation requirement of Tapidor. Other BnaFLC copies show tissue-dependent reactivation of expression post-cold, with these dynamics suggesting some copies have retained or acquired a perennial nature. BnaSOC1 genes, also related to the vernalisation pathway, have expression profiles which suggest tissue subfunctionalisation. This understanding may help to breed varieties with more consistent or robust vernalisation responses, of special importance due to the milder winters resulting from climate change.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brassica napus Language: En Journal: Sci Rep Year: 2024 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brassica napus Language: En Journal: Sci Rep Year: 2024 Document type: Article Country of publication: United kingdom