Supported Molecular Matrix Electrophoresis.
Methods Mol Biol
; 2763: 79-97, 2024.
Article
in En
| MEDLINE
| ID: mdl-38347402
ABSTRACT
Distinct bands of mucins cannot be banded using a gel electrophoresis based on a molecular sieving effect due to their very large molecular weight and remarkable diversity in glycosylation. In contrast, membrane electrophoresis can separate mucins as round bands. Here, we present an analysis of mucin separation via membrane electrophoresis using a porous polyvinylidene difluoride membrane, which is highly stable against chemical modifications and various organic solvents. The separated mucins can not only be stained with dyes but also with antibodies and lectins, and glycans can be released from the excised bands and analyzed.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Coloring Agents
/
Mucins
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Japan
Country of publication:
United States