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Measurement of Mucinase Activity.
Tanabe, Hiroki; Morita, Tatsuya; Nishimura, Naomichi.
Affiliation
  • Tanabe H; Department of Nutritional Sciences, Faculty of Health and Welfare Science, Nayoro City University, Hokkaido, Japan.
  • Morita T; College of Agriculture, Academic Institute, Shizuoka University, Shizuoka, Japan.
  • Nishimura N; College of Agriculture, Academic Institute, Shizuoka University, Shizuoka, Japan. nishimura.naomichi@shizuoka.ac.jp.
Methods Mol Biol ; 2763: 345-352, 2024.
Article in En | MEDLINE | ID: mdl-38347424
ABSTRACT
Mucinase consists of some proteases, glycosidases, sulfatases, and sialidases. It is not practical to measure individual enzyme activities when measuring mucinase activity. In this method, mucinase activity is measured using porcine gastric mucin as a substrate and feces as an enzyme source. This description includes fecal pellet preparation, reaction procedure of mucinase, measurement of reducing sugars liberated during the procedure, and determination of nitrogen content in the fecal preparations.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polysaccharide-Lyases / Glycoside Hydrolases Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Japan Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polysaccharide-Lyases / Glycoside Hydrolases Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Japan Country of publication: United States