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Secretory production and characterization of a highly effective chitosanase from Streptomyces coelicolor A3(2) M145 in Pichia pastoris.
Liang, Jiayu; He, Shengbin; Sun, Jian; Bao, Haodong; Cui, Lanyu.
Affiliation
  • Liang J; Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Colleges and Universities, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
  • He S; Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Colleges and Universities, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
  • Sun J; Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Colleges and Universities, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
  • Bao H; Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Colleges and Universities, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
  • Cui L; Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Colleges and Universities, Guangxi Medical University, Nanning, Guangxi, People's Republic of China.
Biotechnol J ; 19(2): e2300402, 2024 Feb.
Article in En | MEDLINE | ID: mdl-38403403
ABSTRACT
In this study, a glycoside hydrolase family 46 chitosanase from Streptomyces coelicolor A3(2) M145 was firstly cloned and expressed in Pichia pastoris GS115 (P. pastoris GS115). The recombinant enzyme (CsnA) showed maximal activity at pH 6.0 and 65°C. Both thermal stability and pH stability of CsnA expressed in P. pastoris GS115 were significantly increased compared with homologous expression in Streptomyces coelicolor A3(2). A stable chitosanase activity of 725.7 ± 9.58 U mL-1 was obtained in fed-batch fermentation. It's the highest level of CsnA from Streptomyces coelicolor expressed in P. pastoris so far. The hydrolytic process of CsnA showed a time-dependent manner. Chitosan oligosaccharides (COSs) generated by CsnA showed antifungal activity against Fusarium oxysporum sp. cucumerinum (F. oxysporum sp. cucumerinum). The secreted expression and hydrolytic performance make the enzyme a desirable biocatalyst for industrial controllable production of chitooligosaccharides with specific degree of polymerization, which have potential to control fungi that cause important crop diseases.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptomyces coelicolor / Saccharomycetales Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptomyces coelicolor / Saccharomycetales Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Country of publication: Germany