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Amphiphilic multi-targeting copolymer micelles efficiently deliver pZNF580 to promote endothelial cell proliferation and migration.
Li, Chen; Xu, Qirong; Meng, Xiangyan; Duo, Xinghong; Feng, Yakai.
Affiliation
  • Li C; School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, Xining, Qinghai 810007, P. R. China. wlkdxh@tju.edu.cn.
  • Xu Q; Key Laboratory of National Ethnic Affairs Commission of Resource Chemistry and Ecological Environment Protection on Qinghai-Tibet Plateau, Xining, Qinghai 810007, P. R. China.
  • Meng X; School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, Xining, Qinghai 810007, P. R. China. wlkdxh@tju.edu.cn.
  • Duo X; Key Laboratory of National Ethnic Affairs Commission of Resource Chemistry and Ecological Environment Protection on Qinghai-Tibet Plateau, Xining, Qinghai 810007, P. R. China.
  • Feng Y; Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin 300072, P. R. China.
J Mater Chem B ; 12(11): 2843-2854, 2024 Mar 13.
Article in En | MEDLINE | ID: mdl-38412450
ABSTRACT
Cationic copolymers are widely used in gene delivery as a non-viral gene vector, but their applications are limited by low transfection efficiency and high cytotoxicity. In order to enhance the transfection efficiency of copolymer micelles to endothelial cells (HUVECs) and reduce their cytotoxicity, this study synthesized an amphipathic multi-targeted copolymer micelle delivery system PCLMD-PPEGMA-NLS-TAT-REDV (TCMs). Gel test results showed that TCMs showed good pZNF580 binding ability and could effectively load the pZNF580 plasmid. The CCK-8 results show that when the concentration of TCMs is greater than 60 µg mL-1, it will affect cell viability and have low cytotoxicity. We found that the multi-targeted copolymer micelles can be effectively taken up by HUVECs in vitro. The transfection efficiency of TCMs@pZNF580 (w/wpZNF580 = 3) to HUVECs was comparable to that of the positive control group lip2000@pZNF580, and WB also showed the same trend. In addition, the TCMs@pZNF580 complex also significantly enhanced the proliferation and migration of HUVECs. The experimental results on blood vessel formation showed that TCMs@pZNF580 accelerated the vascularization of HUVECs. This experiment provided a new technology platform for targeted gene therapy, especially for endothelialization and vascularization. The research results have important reference value for the treatment of cardiovascular diseases.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endothelial Cells / Micelles Limits: Humans Language: En Journal: J Mater Chem B Year: 2024 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Endothelial Cells / Micelles Limits: Humans Language: En Journal: J Mater Chem B Year: 2024 Document type: Article Country of publication: United kingdom