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Clonal Distribution and Its Association With the Carbapenem Resistance Mechanisms of Carbapenem-Non-Susceptible Pseudomonas aeruginosa Isolates From Korean Hospitals.
Kim, Nayeong; Ko, Seo Yeon; Park, Seong Yong; Kim, Seong Yeob; Lee, Da Eun; Kwon, Ki Tae; Kim, Yu Kyung; Lee, Je Chul.
Affiliation
  • Kim N; Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Korea.
  • Ko SY; Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Korea.
  • Park SY; Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Korea.
  • Kim SY; Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Korea.
  • Lee DE; Kyungpook National University Hospital National Culture Collection for Pathogens (KNUH-NCCP), Kyungpook National University Hospital, Daegu, Korea.
  • Kwon KT; Kyungpook National University Hospital National Culture Collection for Pathogens (KNUH-NCCP), Kyungpook National University Hospital, Daegu, Korea.
  • Kim YK; Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Korea.
  • Lee JC; Kyungpook National University Hospital National Culture Collection for Pathogens (KNUH-NCCP), Kyungpook National University Hospital, Daegu, Korea.
Ann Lab Med ; 44(5): 410-417, 2024 Sep 01.
Article in En | MEDLINE | ID: mdl-38433574
ABSTRACT

Background:

Carbapenem resistance in Pseudomonas aeruginosa is a serious global health problem. We investigated the clonal distribution and its association with the carbapenem resistance mechanisms of carbapenem-non-susceptible P. aeruginosa isolates from three Korean hospitals.

Methods:

A total of 155 carbapenem-non-susceptible P. aeruginosa isolates collected between 2011 and 2019 were analyzed for sequence types (STs), antimicrobial susceptibility, and carbapenem resistance mechanisms, including carbapenemase production, the presence of resistance genes, OprD mutations, and the hyperproduction of AmpC ß-lactamase.

Results:

Sixty STs were identified in carbapenem-non-susceptible P. aeruginosa isolates. Two high-risk clones, ST235 (N=41) and ST111 (N=20), were predominant; however, sporadic STs were more prevalent than high-risk clones. The resistance rate to amikacin was the lowest (49.7%), whereas that to piperacillin was the highest (92.3%). Of the 155 carbapenem-non-susceptible isolates, 43 (27.7%) produced carbapenemases. Three metallo-ß-lactamase (MBL) genes, blaIMP-6 (N=38), blaVIM-2 (N=3), and blaNDM-1 (N=2), were detected. blaIMP-6 was detected in clonal complex 235 isolates. Two ST773 isolates carried blaNDM-1 and rmtB. Frameshift mutations in oprD were identified in all isolates tested, regardless of the presence of MBL genes. Hyperproduction of AmpC was detected in MBL gene-negative isolates.

Conclusions:

Frameshift mutations in oprD combined with MBL production or hyperproduction of AmpC are responsible for carbapenem resistance in P. aeruginosa. Further attention is required to curb the emergence and spread of new carbapenem-resistant P. aeruginosa clones.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudomonas aeruginosa / Pseudomonas Infections / Bacterial Proteins / Beta-Lactamases / Microbial Sensitivity Tests / Carbapenems / Hospitals / Anti-Bacterial Agents Limits: Humans Country/Region as subject: Asia Language: En Journal: Ann Lab Med Year: 2024 Document type: Article Country of publication: Korea (South)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudomonas aeruginosa / Pseudomonas Infections / Bacterial Proteins / Beta-Lactamases / Microbial Sensitivity Tests / Carbapenems / Hospitals / Anti-Bacterial Agents Limits: Humans Country/Region as subject: Asia Language: En Journal: Ann Lab Med Year: 2024 Document type: Article Country of publication: Korea (South)