A mouse DRG genetic toolkit reveals morphological and physiological diversity of somatosensory neuron subtypes.
Cell
; 187(6): 1508-1526.e16, 2024 Mar 14.
Article
in En
| MEDLINE
| ID: mdl-38442711
ABSTRACT
Dorsal root ganglia (DRG) somatosensory neurons detect mechanical, thermal, and chemical stimuli acting on the body. Achieving a holistic view of how different DRG neuron subtypes relay neural signals from the periphery to the CNS has been challenging with existing tools. Here, we develop and curate a mouse genetic toolkit that allows for interrogating the properties and functions of distinct cutaneous targeting DRG neuron subtypes. These tools have enabled a broad morphological analysis, which revealed distinct cutaneous axon arborization areas and branching patterns of the transcriptionally distinct DRG neuron subtypes. Moreover, in vivo physiological analysis revealed that each subtype has a distinct threshold and range of responses to mechanical and/or thermal stimuli. These findings support a model in which morphologically and physiologically distinct cutaneous DRG sensory neuron subtypes tile mechanical and thermal stimulus space to collectively encode a wide range of natural stimuli.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sensory Receptor Cells
/
Ganglia, Spinal
/
Single-Cell Gene Expression Analysis
Limits:
Animals
Language:
En
Journal:
Cell
Year:
2024
Document type:
Article
Affiliation country:
United States