Using synthetic genome readers/regulators to interrogate chromatin processes: A brief review.
Methods
; 225: 20-27, 2024 May.
Article
in En
| MEDLINE
| ID: mdl-38471600
ABSTRACT
Aberrant gene expression underlies numerous human ailments. Hence, developing small molecules to target and remedy dysfunctional gene regulation has been a long-standing goal at the interface of chemistry and medicine. A major challenge for designing small molecule therapeutics aimed at targeting desired genomic loci is the minimization of widescale disruption of genomic functions. To address this challenge, we rationally design polyamide-based multi-functional molecules, i.e., Synthetic Genome Readers/Regulators (SynGRs), which, by design, target distinct sequences in the genome. Herein, we briefly review how SynGRs access chromatin-bound and chromatin-free genomic sites, then highlight the methods for the study of chromatin processes using SynGRs on positioned nucleosomes in vitro or disease-causing repressive genomic loci in vivo.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatin
/
Nucleosomes
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods
Journal subject:
BIOQUIMICA
Year:
2024
Document type:
Article
Affiliation country:
United States