Application of Thermomyces lanuginosus polygalacturonase produced in Komagataella phaffii in biomass hydrolysis and textile bioscouring.
Enzyme Microb Technol
; 177: 110424, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38479075
ABSTRACT
In this work, the polygalacturonase (TL-PG1) from the thermophilic fungus Thermomyces lanuginosus was heterologously produced for the first time in the yeast Komagataella phaffii. The TL-PG1 was successfully expressed under the control of the AOX1 promoter and sequentially purified by His-tag affinity. The purified recombinant pectinase exhibited an activity of 462.6â¯U/mL toward polygalacturonic acid under optimal conditions (pH 6 and 55 ËC) with a 2.83â¯mg/mL and 0.063 µmol/minute for Km and Vmax, respectively. When used as supplementation for biomass hydrolysis, TL-PG1 demonstrated synergy with the enzymatic cocktail Ctec3 to depolymerize orange citrus pulp, releasing 1.43â¯mg/mL of reducing sugar. In addition, TL-PG1 exhibited efficiency in fabric bioscouring, showing potential usage in the textile industry. Applying a protein dosage of 7â¯mg/mL, the time for the fabric to absorb water was 19.77â¯seconds (ten times faster than the control). Adding the surfactant Triton to the treatment allowed the reduction of the enzyme dosage by 50% and the water absorption time to 6.38â¯seconds. Altogether, this work describes a new versatile polygalacturonase from T. lanuginosus with the potential to be employed in the hydrolysis of lignocellulosic biomass and bioscouring.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polygalacturonase
/
Fungal Proteins
/
Saccharomycetales
Language:
En
Journal:
Enzyme Microb Technol
Year:
2024
Document type:
Article
Affiliation country:
Brazil