Azoanthracene-core structure as Cu2+-assisted CO sensing probe: Characterization, performance, and bioimaging.
Spectrochim Acta A Mol Biomol Spectrosc
; 313: 124122, 2024 May 15.
Article
in En
| MEDLINE
| ID: mdl-38479230
ABSTRACT
Detection of endogenous CO (carbon monoxide) is an interesting topic in biology because it has been discovered as a messenger for signal transduction and therapeutic effects in vital biological activities. Fluorescence imaging has proven a powerful tool for detecting endogenous CO, which drives the development of low-cost and easy-to-use fluorescent probes. In this study, four azobenzene derivatives (A1, A2, A3, and A4) with various substituents were reported, including their geometric structures, photophysical parameters, and spectral responses to Cu2+ and CO. The relationship between substituent structure and performance was discussed along with Cu2+ quenching and CO sensing mechanisms. The optimal probe (A1), which had no substituent, efficiently quenched fluorescence in the presence of Cu2+, with its PLQY decreased from 0.33 to 0.02, PLQY = photoluminescence quantum yield. Upon CO deoxidization, A1's fluorescence could be recovered (PLQY recovered to 0.32) within 180 s. Its sensing mechanism was static by forming a non-fluorescent complex with Cu2+ (with a stoichiometric ratio of 11). The bioimaging performance of A1 for endogenous CO in HeLa cells was reported.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Copper
/
Fluorescent Dyes
Limits:
Humans
Language:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United kingdom