Establishment and application of recombinase polymerase amplification combined with a lateral flow dipstick for the detection of mcr-1 in uncultured clinical samples.
Int J Antimicrob Agents
; 63(5): 107140, 2024 May.
Article
in En
| MEDLINE
| ID: mdl-38490574
ABSTRACT
OBJECTIVES:
The rapid dissemination of the mcr-1 gene via plasmid-mediated transfer has raised concerns regarding the efficacy of colistin as a last-resort treatment for multidrug-resistant Gram-negative bacterial infections. Current mcr-1 gene detection methods mainly focus on cultured bacteria, which is a complex and time-consuming process requiring skilled personnel, making it unsuitable for field analysis.METHODS:
A rapid detection technique combining recombinase polymerase amplification with a lateral flow dipstick targeting uncultured clinical samples was developed.RESULTS:
This new method targeting the mcr-1 gene region (23 232-23 642 bp, no. KP347127.1) achieved a low detection limit of 10 copies/µL. The whole process was carried out with high specificity and was completed within 20 min. The evaluation assay was conducted using 45 human faecal samples; 16 strains yielded a 98% accuracy, closely matching antimicrobial susceptibility outcomes.CONCLUSIONS:
The novel method integrates nucleic acid extraction, isothermal amplification, and a test assay, suggesting the potential for timely colistin resistance surveillance in frontline disease control and healthcare settings, supporting future prevention and clinical standardization efforts.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Nucleic Acid Amplification Techniques
/
Drug Resistance, Bacterial
/
Enterobacteriaceae
/
Ethanolaminephosphotransferase
Limits:
Humans
Language:
En
Journal:
Int J Antimicrob Agents
Year:
2024
Document type:
Article
Affiliation country:
China