Modulatory effects of Moringa oleifera leaf extract on sodium nitrate-induced experimental colitis via regulation of P53, Ki-67 and PCNA biomarkers.
Tissue Cell
; 88: 102327, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38493756
ABSTRACT
BACKGROUND:
Ulcerative colitis is a risk factor for colorectal carcinoma. Different mechanisms are related to colitis like apoptosis and hyperproliferation. Moringa oleifera leaves extract (MO) provides a promising option to overcome the risk.PURPOSE:
To examine the colonic changes in a rat model of colitis induced by sodium nitrate (SN) and study the effects of MO. STUDYDESIGN:
Eight adult male rats were allocated in each of the three group; control (distilled water), SN (100â¯mg/kg/day, orally via gastric gavage), and SN + MO (100â¯mg/kg/day, orally via gastric gavage).METHODS:
Body weight was measured after the end of the experiment. Colonic homogenates were tested for levels of oxidative stress indicators. Immunohistochemistry for P53, PCNA and Ki-67 was performed. Fresh colon specimens were used for quantitative real-time PCR for assessment of P53, PCNA and Ki-67 gene expression.RESULTS:
SN group revealed a significant decreased weight (p = 0.002). MDA and NO levels were higher with SN administration than with MO co-administration (p= 0.04, 0.01 respectively). GSH level was reduced in SN group (p = 0.02) and significantly increased with MO intake (p = 0.04). SN-induced colonic destructive changes were reversed with MO. P53, PCNA and Ki-67 levels of gene expression were reduced in SN + MO group than SN group (P = 0.007, 0.02, 0.001 respectively).CONCLUSION:
MO protected the colonic mucosa against SN-induced changes regulating apoptosis, and cell proliferation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Extracts
/
Tumor Suppressor Protein p53
/
Proliferating Cell Nuclear Antigen
/
Plant Leaves
/
Ki-67 Antigen
/
Moringa oleifera
/
Nitrates
Limits:
Animals
Language:
En
Journal:
Tissue Cell
Year:
2024
Document type:
Article
Country of publication:
United kingdom