All-optical presynaptic plasticity induction by photoactivated adenylyl cyclase targeted to axon terminals.
Cell Rep Methods
; 4(4): 100740, 2024 Apr 22.
Article
in En
| MEDLINE
| ID: mdl-38521059
ABSTRACT
Intracellular signaling plays essential roles in various cell types. In the central nervous system, signaling cascades are strictly regulated in a spatiotemporally specific manner to govern brain function; for example, presynaptic cyclic adenosine monophosphate (cAMP) can enhance the probability of neurotransmitter release. In the last decade, channelrhodopsin-2 has been engineered for subcellular targeting using localization tags, but optogenetic tools for intracellular signaling are not well developed. Therefore, we engineered a selective presynaptic fusion tag for photoactivated adenylyl cyclase (bPAC-Syn1a) and found its high localization at presynaptic terminals. Furthermore, an all-optical electrophysiological method revealed rapid and robust short-term potentiation by bPAC-Syn1a at brain stem-amygdala synapses in acute brain slices. Additionally, bPAC-Syn1a modulated mouse immobility behavior. These results indicate that bPAC-Syn1a can manipulate presynaptic cAMP signaling in vitro and in vivo. The all-optical manipulation technique developed in this study can help further elucidate the dynamic regulation of various cellular functions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenylyl Cyclases
/
Presynaptic Terminals
/
Cyclic AMP
/
Neuronal Plasticity
Limits:
Animals
Language:
En
Journal:
Cell Rep Methods
Year:
2024
Document type:
Article
Affiliation country:
Japan