Roles for CEP170 in cilia function and dynein-2 assembly.
J Cell Sci
; 137(8)2024 04 15.
Article
in En
| MEDLINE
| ID: mdl-38533689
ABSTRACT
Primary cilia are essential eukaryotic organelles required for signalling and secretion. Dynein-2 is a microtubule-motor protein complex and is required for ciliogenesis via its role in facilitating retrograde intraflagellar transport (IFT) from the cilia tip to the cell body. Dynein-2 must be assembled and loaded onto IFT trains for entry into cilia for this process to occur, but how dynein-2 is assembled and how it is recycled back into a cilium remain poorly understood. Here, we identify centrosomal protein of 170â
kDa (CEP170) as a dynein-2-interacting protein in mammalian cells. We show that loss of CEP170 perturbs intraflagellar transport and hedgehog signalling, and alters the stability of dynein-2 holoenzyme complex. Together, our data indicate a role for CEP170 in supporting cilia function and dynein-2 assembly.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cilia
/
Microtubule-Associated Proteins
Limits:
Animals
/
Humans
Language:
En
Journal:
J Cell Sci
Year:
2024
Document type:
Article
Country of publication:
United kingdom