Linear dichroism reveals the perpendicular orientation of DNA bases in the RecA and Rad51 recombinase filaments: A possible mechanism for the strand exchange reaction.
Chirality
; 36(4): e23664, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38561319
ABSTRACT
Linear dichroism spectroscopy is used to investigate the structure of RecA family recombinase filaments (RecA and Rad51 proteins) with DNA for clarifying the molecular mechanism of DNA strand exchange promoted by these proteins and its activation. The measurements show that the recombinases promote the perpendicular base orientation of single-stranded DNA only in the presence of activators, indicating the importance of base orientation in the reaction. We summarize the results and discuss the role of DNA base orientation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Rad51 Recombinase
Language:
En
Journal:
Chirality
Journal subject:
BIOLOGIA MOLECULAR
/
QUIMICA
Year:
2024
Document type:
Article
Affiliation country:
Japan
Country of publication:
United States